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KMID : 0545120200300020296
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Journal of Microbiology and Biotechnology
2020 Volume.30 No. 2 p.296 ~ p.305
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Biosynthesis of (R)-(-)-1-Octen-3-ol in Recombinant Saccharomyces cerevisiae with Lipoxygenase-1 and Hydroperoxide Lyase Genes from Tricholoma matsutake
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Lee Nan-Yeong
Choi Doo-Ho
Kim Mi-Gyeong
Jeong Min-Ji
Kwon Hae-Jun
Kim Dong-Hyun
Kim Young-Guk
di Luccio Eric
Arioka Manabu
Yoon Hyeok-Jun
Kim Jong-Guk
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Abstract
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Tricholoma matsutake is an ectomycorrhizal fungus, related with the host of Pinus densiflora. Most of studies on T. matsutake have focused on mycelial growth, genes and genomics, phylogenetics, symbiosis, and immune activity of this strain. T. matsutake is known for its unique fragrance in Eastern Asia. The most major component of its scent is (R)-(-)-1-octen-3-ol and is biosynthesized from the substrate linoleic acid by the sequential reaction of lipoxygenase and peroxide lyase. Here, we report for the first time the biosynthesis of (R)-(-)- 1-octen-3-ol of T. matsutake using the yeast Saccharomyces cerevisiae as a host. In this study, cDNA genes correlated with these reactions were cloned from T. matsutake, and expression studies of theses genes were carried out in the yeast Saccharomyces cerevisiae. The product of these genes expression study was carried out with Western blotting. The biosynthesis of (R)-(-)- 1-octen-3-ol of T. matsutake in recombinant Saccharomyces cerevisiae was subsequently identified with GC-MS chromatography analysis. The biosynthesis of (R)-(-)-1-octen-3-ol with S. cerevisiae represents a significant step forward.
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KEYWORD
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(R)-(-)-1-octen-3-ol, Tricholoma matsutake, lipoxygenase, hydroperoxide lyase, Saccharomyces cerevisiae
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